Implementation of the research agenda
The company began implementing the project on January 1, 2023. The project was innovative in nature due to its objectives—to develop formulations containing a minimal amount of active ingredient while maintaining efficacy against microorganisms, including antibiotic-resistant bacteria from the ESKAPE group and viruses. The ESKAPE group of bacteria includes, among others, Enterococcus spp., Staphylococcus aureus, Klebsiella spp., Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp., which are largely responsible for hospital-acquired infections.
The project work was carried out using high-quality laboratory equipment. During the research, suitable raw materials were successfully identified, including nonionic and anionic surfactants as well as alkyl polyglucosides, on the basis of which numerous cleaning formulations were developed.
All raw materials were thoroughly analyzed for their physicochemical properties and then evaluated according to an established matrix, taking into account, among other factors, CMC, biodegradability, availability, potential harm to humans, and environmental impact. This allowed for the selection of the most suitable components for further research and development.
After selecting the target surfactants, the most effective cleaning formulations were developed, based exclusively on surfactants without the addition of auxiliary substances, so that they met all the project’s design requirements. This stage was successful—base cleaning systems were selected, which, after yielding positive stability test results, were further tested for effectiveness against biofilm. They were evaluated both in terms of their ability to remove bacterial biofilm and to limit its formation.
As a result of the research, formulations were developed containing an active ingredient at a level below 12 g/100 g (calculated as pure raw material). They demonstrated an effect that prevents the formation of bacterial biofilm. As part of the project, the formulations were reformulated several times to achieve the best possible performance and microbiological results. For the selected formulations, positive results were obtained, confirming their effectiveness in preventing biofilm formation.
The project also determined the performance properties of the formulations regarding the removal of biofilm and organic contaminants, as well as the properties of the cleaning and disinfecting systems in terms of bactericidal efficacy. Additionally, optimal forms of product application were determined, its stability was confirmed, compatibility with materials was assessed, and other physical and chemical properties were characterized, including aspects related to hazardous substances, toxicological safety, and ecological impact.
